Simultaneously monitoring DNA binding and helicase-catalyzed DNA unwinding by fluorescence polarization

A new method for helicase-catalyzed DNA unwinding is described. This assay takes advantage of the substantial change in fluorescence polarization (FP) upon helicase binding and DNA unwinding. The low anisotropy value, due to the fast tumbling of the free oligonucleotide in solution, increases abrupt...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Xu, H. Q., Zhang, A. H., Auclair, C., Xi, X. G.
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2003
Matèries:	NAR Methods Online
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167644/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12853647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng070
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