Simultaneously monitoring DNA binding and helicase-catalyzed DNA unwinding by fluorescence polarization
A new method for helicase-catalyzed DNA unwinding is described. This assay takes advantage of the substantial change in fluorescence polarization (FP) upon helicase binding and DNA unwinding. The low anisotropy value, due to the fast tumbling of the free oligonucleotide in solution, increases abrupt...
में बचाया:
| में प्रकाशित: | Nucleic Acids Res |
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| मुख्य लेखकों: | , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Oxford University Press
2003
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167644/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12853647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng070 |
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