Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn917-lacZ integrants with the new promoter probe vector, pAK80.

Transposon Tn917-LTV1 was used to produce a collection of Lactococcus lactis strains with fusion of a promoterless lacZ gene to chromosomal loci. Screening 2,500 Tn917-LTV1 integrants revealed 222 that express beta-galactosidase on plates at 30 degrees C. Pulsed-field gel electrophoresis revealed Tn...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Israelsen, H, Madsen, S M, Vrang, A, Hansen, E B, Johansen, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167525/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7618865/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.7.2540-2547.1995
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