Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum.

The major cellulose-binding domain (CBD) from the cellulosome of Clostridium thermocellum YS was cloned and overexpressed in Escherichia coli. The expressed protein was purified efficiently by a modification of a novel procedure termed affinity digestion. The properties of the purified polypeptide w...

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Bibliographic Details
Main Authors: Morag, E, Lapidot, A, Govorko, D, Lamed, R, Wilchek, M, Bayer, E A, Shoham, Y
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC167460/
https://ncbi.nlm.nih.gov/pubmed/7646033
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