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Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum.

The major cellulose-binding domain (CBD) from the cellulosome of Clostridium thermocellum YS was cloned and overexpressed in Escherichia coli. The expressed protein was purified efficiently by a modification of a novel procedure termed affinity digestion. The properties of the purified polypeptide w...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Appl Environ Microbiol
Prif Awduron: Morag, E, Lapidot, A, Govorko, D, Lamed, R, Wilchek, M, Bayer, E A, Shoham, Y
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 1995
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167460/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7646033/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.5.1980-1986.1995
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