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Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product.

Flow cytometry is a rapid and sensitive method which may be used for the detection of microorganisms in foods and drinks. A key requirement for this method is a sufficient fluorescence staining of the target cells. The mechanism of staining of the yeast Saccharomyces cerevisiae by fluorescein diacet...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Breeuwer, P, Drocourt, J L, Bunschoten, N, Zwietering, M H, Rombouts, F M, Abee, T
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167417/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7747975/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.4.1614-1619.1995
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