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Reverse transcription PCR to detect enteroviruses in surface water.

We have developed a simple, fast, and efficient procedure to detect enteroviruses in water samples. Aliquots of water are subjected to two-step filtration, with the second filter containing a positively charged nylon membrane that holds back virus particles. Viruses thus adsorbed are directly lysed,...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Gilgen, M, Wegmüller, B, Burkhalter, P, Bühler, H P, Müller, U, Lüthy, J, Candrian, U
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1995
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167377/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7747945/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.4.1226-1231.1995
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