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Reverse transcription PCR to detect enteroviruses in surface water.
We have developed a simple, fast, and efficient procedure to detect enteroviruses in water samples. Aliquots of water are subjected to two-step filtration, with the second filter containing a positively charged nylon membrane that holds back virus particles. Viruses thus adsorbed are directly lysed,...
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Main Authors: | , , , , , , |
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Format: | Artigo |
Language: | Inglês |
Published: |
1995
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC167377/ https://ncbi.nlm.nih.gov/pubmed/7747945 |
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