Reverse transcription PCR to detect enteroviruses in surface water.

We have developed a simple, fast, and efficient procedure to detect enteroviruses in water samples. Aliquots of water are subjected to two-step filtration, with the second filter containing a positively charged nylon membrane that holds back virus particles. Viruses thus adsorbed are directly lysed,...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Gilgen, M, Wegmüller, B, Burkhalter, P, Bühler, H P, Müller, U, Lüthy, J, Candrian, U
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1995
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167377/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7747945/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.4.1226-1231.1995
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