A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2-...

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Bibliografski detalji

Izdano u:	Appl Environ Microbiol
Glavni autori:	Loessner, M J, Schneider, A, Scherer, S
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1995
Teme:	Research Article
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7540820/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.3.1150-1152.1995
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