A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2-...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Loessner, M J, Schneider, A, Scherer, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7540820/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.3.1150-1152.1995
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