Possible errors in assay for beta-glycosidase activity.

Cecal homogenates were assayed for the enzymes beta-glucosidase, beta-glucuronidase, and beta-galactosidase. Anaerobic incubation with the addition of excess 3,4-dichloronitrobenzene, a substrate for nitroreductase, significantly increased the detection of the beta-glycosidase enzymes' activities.

Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Chadwick, R W, Allison, J C, Talley, D L, George, S E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167344/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7574621/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.2.820-822.1995
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