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Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.

A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction al...

詳細記述

保存先:
書誌詳細
主要な著者: Baez, L A, Juneja, V K
フォーマット: Artigo
言語:Inglês
出版事項: 1995
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC167342/
https://ncbi.nlm.nih.gov/pubmed/7574619
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