Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.

A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction al...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Baez, L A, Juneja, V K
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1995
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167342/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7574619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.2.807-810.1995
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