Active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues.
Two cysteine residues (C-265 and C-318) in the putative hydrophilic regions of sarcosine oxidase were substituted by using site-directed mutagenesis. Since the mutant with the C-to-S mutation at position 318 (C318S) lost the enzyme activity, C-318 (conserved among sarcosine oxidases) is most likely...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Appl Environ Microbiol |
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| Κύριοι συγγραφείς: | , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
American Society for Microbiology (ASM)
1995
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167291/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7887617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.1.367-370.1995 |
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