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Successive Glycosyltransfer Activity and Enzymatic Characterization of Pectic Polygalacturonate 4-α-Galacturonosyltransferase Solubilized from Pollen Tubes of Petunia axillaris Using Pyridylaminated Oligogalacturonates as Substrates
Polygalacturonate 4-α-galacturonosyltransferase (pectin synthase) was solubilized from pollen tubes of Petunia axillaris and characterized. To accomplish this, an assay method using fluorogenic pyridylaminated-oligogalacturonic acids (PA-OGAs) as acceptor substrates was developed. When the pollen tu...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society of Plant Physiologists
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC166569/ https://ncbi.nlm.nih.gov/pubmed/12226516 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.005587 |
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