Successive Glycosyltransfer Activity and Enzymatic Characterization of Pectic Polygalacturonate 4-α-Galacturonosyltransferase Solubilized from Pollen Tubes of Petunia axillaris Using Pyridylaminated Oligogalacturonates as Substrates

Polygalacturonate 4-α-galacturonosyltransferase (pectin synthase) was solubilized from pollen tubes of Petunia axillaris and characterized. To accomplish this, an assay method using fluorogenic pyridylaminated-oligogalacturonic acids (PA-OGAs) as acceptor substrates was developed. When the pollen tu...

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Main Authors: Akita, Kazumasa, Ishimizu, Takeshi, Tsukamoto, Tatsuya, Ando, Toshio, Hase, Sumihiro
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Physiologists 2002
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC166569/
https://ncbi.nlm.nih.gov/pubmed/12226516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.005587
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