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Temperature-Dependent Hypermutational Phenotype in recA Mutants of Thermus thermophilus HB27
The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (ΔrecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2003
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC166453/ https://ncbi.nlm.nih.gov/pubmed/12897010 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.16.4901-4907.2003 |
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