Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures

BACKGROUND: A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut(+ )strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix p...

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Bibliografiske detaljer
Main Authors: Jahic, Mehmedalija, Wallberg, Fredrik, Bollok, Monika, Garcia, Percival, Enfors, Sven-Olof
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2003
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC166161/
https://ncbi.nlm.nih.gov/pubmed/12871597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-2-6
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