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Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation

Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intri...

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Detalhes bibliográficos
Main Authors: Zipfel, Warren R., Williams, Rebecca M., Christie, Richard, Nikitin, Alexander Yu, Hyman, Bradley T., Webb, Watt W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC165832/
https://ncbi.nlm.nih.gov/pubmed/12756303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0832308100
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