Stepping rotation of F(1)-ATPase visualized through angle-resolved single-fluorophore imaging

Orientation dependence of single-fluorophore intensity was exploited in order to videotape conformational changes in a protein machine in real time. The fluorophore Cy3 attached to the central subunit of F(1)-ATPase revealed that the subunit rotates in the molecule in discrete 120° steps and that ea...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Adachi, Kengo, Yasuda, Ryohei, Noji, Hiroyuki, Itoh, Hiroyasu, Harada, Yoshie, Yoshida, Masasuke, Kinosita, Kazuhiko
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16530/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10840052/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.120174297
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!