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Stepping rotation of F(1)-ATPase visualized through angle-resolved single-fluorophore imaging
Orientation dependence of single-fluorophore intensity was exploited in order to videotape conformational changes in a protein machine in real time. The fluorophore Cy3 attached to the central subunit of F(1)-ATPase revealed that the subunit rotates in the molecule in discrete 120° steps and that ea...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
The National Academy of Sciences
2000
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC16530/ https://ncbi.nlm.nih.gov/pubmed/10840052 |
| Oznake: |
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