Stepping rotation of F(1)-ATPase visualized through angle-resolved single-fluorophore imaging

Orientation dependence of single-fluorophore intensity was exploited in order to videotape conformational changes in a protein machine in real time. The fluorophore Cy3 attached to the central subunit of F(1)-ATPase revealed that the subunit rotates in the molecule in discrete 120° steps and that ea...

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Autores principales: Adachi, Kengo, Yasuda, Ryohei, Noji, Hiroyuki, Itoh, Hiroyasu, Harada, Yoshie, Yoshida, Masasuke, Kinosita, Kazuhiko
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences 2000
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC16530/
https://ncbi.nlm.nih.gov/pubmed/10840052
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