Targeted cytosine methylation for in vivo detection of protein–DNA interactions

We report a technique, named targeted gene methylation (TAGM), for identifying in vivo protein-binding sites in chromatin. M.CviPI, a cytosine-5 DNA methyltransferase recognizing GC sites, is fused to a DNA-binding factor enabling simultaneous detection of targeted methylation, factor footprints, an...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Carvin, Christopher D., Dhasarathy, Archana, Friesenhahn, Laurie B., Jessen, Walter J., Kladde, Michael P.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2003
Pynciau:
Biological Sciences
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC164658/
https://ncbi.nlm.nih.gov/pubmed/12808133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1332672100
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg