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Targeted cytosine methylation for in vivo detection of protein–DNA interactions
We report a technique, named targeted gene methylation (TAGM), for identifying in vivo protein-binding sites in chromatin. M.CviPI, a cytosine-5 DNA methyltransferase recognizing GC sites, is fused to a DNA-binding factor enabling simultaneous detection of targeted methylation, factor footprints, an...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC164658/ https://ncbi.nlm.nih.gov/pubmed/12808133 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1332672100 |
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