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How the BfiI restriction enzyme uses one active site to cut two DNA strands

Unlike other restriction enzymes, BfiI functions without metal ions. It recognizes an asymmetric DNA sequence, 5′-ACTGGG-3′, and cuts top and bottom strands at fixed positions downstream of this sequence. Many restriction enzymes are dimers of identical subunits, with one active site for each DNA st...

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Bibliografiske detaljer
Main Authors: Sasnauskas, Giedrius, Halford, Stephen E., Siksnys, Virginijus
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC164460/
https://ncbi.nlm.nih.gov/pubmed/12750473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1131003100
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