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How the BfiI restriction enzyme uses one active site to cut two DNA strands
Unlike other restriction enzymes, BfiI functions without metal ions. It recognizes an asymmetric DNA sequence, 5′-ACTGGG-3′, and cuts top and bottom strands at fixed positions downstream of this sequence. Many restriction enzymes are dimers of identical subunits, with one active site for each DNA st...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC164460/ https://ncbi.nlm.nih.gov/pubmed/12750473 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1131003100 |
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