A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners

We developed a retrovirus-based protein-fragment complementation assay (RePCA) screen to identify protein–protein interactions in mammalian cells. In RePCA, bait protein is fused to one fragment of a rationally dissected fluorescent protein, such as GFP, intensely fluorescent protein, or red fluores...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Ding, Zhiyong, Liang, Jiyong, Lu, Yiling, Yu, Qinghua, Songyang, Zhou, Lin, Shiaw-Yih, Mills, Gordon B.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2006
Pynciau:
Biological Sciences
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636760/
https://ncbi.nlm.nih.gov/pubmed/17018644
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0606917103
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