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A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners
We developed a retrovirus-based protein-fragment complementation assay (RePCA) screen to identify protein–protein interactions in mammalian cells. In RePCA, bait protein is fused to one fragment of a rationally dissected fluorescent protein, such as GFP, intensely fluorescent protein, or red fluores...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1636760/ https://ncbi.nlm.nih.gov/pubmed/17018644 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0606917103 |
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