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In vitro evolution of single-chain antibodies using mRNA display
Here we describe the application of the in vitro virus mRNA display method, which involves covalent linkage of an in vitro-synthesized antibody (phenotype) to its encoding mRNA (genotype) through puromycin, for in vitro evolution of single-chain Fv (scFv) antibody fragments. To establish the validit...
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| 主要な著者: | , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2006
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1636464/ https://ncbi.nlm.nih.gov/pubmed/17012279 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl618 |
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