Llwytho...

Dynamics of Single DNA Looping and Cleavage by Sau3AI and Effect of Tension Applied to the DNA

Looping and cleavage of single DNA molecules by the two-site restriction endonuclease Sau3AI were measured with optical tweezers. A DNA template containing many recognition sites was used, permitting loop sizes from ∼10 to 10,000 basepairs. At high enzyme concentration, cleavage events were detected...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Gemmen, Gregory J., Millin, Rachel, Smith, Douglas E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Biophysical Society 2006
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635689/
https://ncbi.nlm.nih.gov/pubmed/16963513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088518
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