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Dynamics of Single DNA Looping and Cleavage by Sau3AI and Effect of Tension Applied to the DNA
Looping and cleavage of single DNA molecules by the two-site restriction endonuclease Sau3AI were measured with optical tweezers. A DNA template containing many recognition sites was used, permitting loop sizes from ∼10 to 10,000 basepairs. At high enzyme concentration, cleavage events were detected...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Biophysical Society
2006
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1635689/ https://ncbi.nlm.nih.gov/pubmed/16963513 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088518 |
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