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Cloning, sequencing, and site-directed mutagenesis of beta-lactamase gene from Streptomyces fradiae Y59.

The beta-lactamase gene from Streptomyces fradiae Y59 was cloned and sequenced. To determine which amino acid residues are critical in binding activity to blue dextran, chimera beta-lactamases were constructed and their binding abilities were determined. The results suggested that blue dextran bindi...

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Bibliographic Details
Main Authors: Kurai, S, Urabe, H, Ogawara, H
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC162522/
https://ncbi.nlm.nih.gov/pubmed/7535038
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