Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry

The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dyna...

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Dades bibliogràfiques
Autors principals: De Medici, Dario, Croci, Luciana, Delibato, Elisabetta, Di Pasquale, Simona, Filetici, Emma, Toti, Laura
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2003
Matèries:
Food Microbiology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC161507/
https://ncbi.nlm.nih.gov/pubmed/12788750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.6.3456-3461.2003
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