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Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry

The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dyna...

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Detalhes bibliográficos
Main Authors: De Medici, Dario, Croci, Luciana, Delibato, Elisabetta, Di Pasquale, Simona, Filetici, Emma, Toti, Laura
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC161507/
https://ncbi.nlm.nih.gov/pubmed/12788750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.6.3456-3461.2003
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