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Method for Regulated Expression of Single-Copy Efflux Pump Genes in a Surrogate Pseudomonas aeruginosa Strain: Identification of the BpeEF-OprC Chloramphenicol and Trimethoprim Efflux Pump of Burkholderia pseudomallei 1026b

Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkhold...

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Detalhes bibliográficos
Main Authors: Kumar, Ayush, Chua, Kim-Lee, Schweizer, Herbert P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1610100/
https://ncbi.nlm.nih.gov/pubmed/17005832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00440-06
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