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Functional expression of Arabidopsis thaliana anthranilate synthase subunit I in Escherichia coli.

Anthranilate synthase is involved in tryptophan (Trp) biosynthesis. Functional expression of subunit I from Arabidopsis (ASA1) was achieved in bacteria as a protein fused with glutathione S-transferase (GST). The active product was purified in a single step on a glutathione-Sepharose column. The Vma...

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Udgivet i:Plant Physiol
Principais autores: Bernasconi, P, Walters, E W, Woodworth, A R, Siehl, D L, Stone, T E, Subramanian, M V
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC159533/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7972519/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.106.1.353
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