Functional expression of Arabidopsis thaliana anthranilate synthase subunit I in Escherichia coli.
Anthranilate synthase is involved in tryptophan (Trp) biosynthesis. Functional expression of subunit I from Arabidopsis (ASA1) was achieved in bacteria as a protein fused with glutathione S-transferase (GST). The active product was purified in a single step on a glutathione-Sepharose column. The Vma...
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| Publicado no: | Plant Physiol |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC159533/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7972519/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.106.1.353 |
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