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High-Throughput Identification of Clinical Pathogenic Fungi by Hybridization to an Oligonucleotide Microarray

Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product...

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Detalhes bibliográficos
Main Authors: Huang, Aihua, Li, Jun-Wen, Shen, Zhi-Qiang, Wang, Xin-Wei, Jin, Min
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1594736/
https://ncbi.nlm.nih.gov/pubmed/16954264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00417-06
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