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Zn(2+)-Dependent Deoxyribozymes That Form Natural and Unnatural RNA Linkages
We report Zn(2+)-dependent deoxyribozymes that ligate RNA. The DNA enzymes were identified by in vitro selection and ligate RNA with k(obs) up to 0.5 min(−1) at 1 mM Zn(2+) and 23 °C, pH 7.9, which is substantially faster than our previously reported Mg(2+)-dependent deoxyribozymes. Each new Zn(2+)-...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1586068/ https://ncbi.nlm.nih.gov/pubmed/15966746 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi050146g |
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