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Zn(2+)-Dependent Deoxyribozymes That Form Natural and Unnatural RNA Linkages

We report Zn(2+)-dependent deoxyribozymes that ligate RNA. The DNA enzymes were identified by in vitro selection and ligate RNA with k(obs) up to 0.5 min(−1) at 1 mM Zn(2+) and 23 °C, pH 7.9, which is substantially faster than our previously reported Mg(2+)-dependent deoxyribozymes. Each new Zn(2+)-...

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Bibliografiset tiedot
Päätekijät: Hoadley, Kelly A., Purtha, Whitney E., Wolf, Amanda C., Flynn-Charlebois, Amber, Silverman, Scott K.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2005
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Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC1586068/
https://ncbi.nlm.nih.gov/pubmed/15966746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi050146g
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