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Liposome-mediated RNA transfection should be used with caution

Liposome-mediated RNA transfection appears to present a number of advantages for studying the metabolism of reporter mRNAs in mammalian cells. This method is also widely used to transfect siRNAs. Here we describe results indicating that reporter mRNAs introduced into HeLa cells by liposomes do not p...

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Main Authors: Barreau, Carine, Dutertre, Stéphanie, Paillard, Luc, Osborne, H. Beverley
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2006
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1581979/
https://ncbi.nlm.nih.gov/pubmed/16921069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.191706
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