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Crystallization of hepatocyte nuclear factor 1β in complex with DNA

Hepatocyte nuclear factor 1β (HNF1β) is a member of the POU transcription-factor family and binds the target DNA as a dimer with nanomolar affinity. The HNF1β–DNA complex has been prepared and crystallized by hanging-drop vapor diffusion in 6%(v/v) PEG 300, 5%(w/v) PEG 8000, 8%(v/v) glycerol and 0.1...

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Detalhes bibliográficos
Main Authors: Lu, Peng, Li, Yun, Gorman, Amanda, Chi, Young-In
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1581457/
https://ncbi.nlm.nih.gov/pubmed/16754972
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106015168
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