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Dissecting Elastic Heterogeneity along DNA Molecules Coated Partly with Rad51 Using Concurrent Fluorescence Microscopy and Optical Tweezers

Nucleoprotein filament formation by recombinases is central to homologous recombination. To follow this process, we used fluorescent human Rad51 recombinase to visualize the interactions with double-stranded DNA (dsDNA). Fluorescence imaging revealed that Rad51 filament formation on dsDNA initiates...

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Detalhes bibliográficos
Main Authors: Mameren, Joost van, Modesti, Mauro, Kanaar, Roland, Wyman, Claire, Wuite, Gijs J. L., Peterman, Erwin J. G.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1578474/
https://ncbi.nlm.nih.gov/pubmed/16920830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.089466
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