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Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

BACKGROUND: Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements. However, successful quantification depends crucially on the...

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Autori principali: Cankar, Katarina, Štebih, Dejan, Dreo, Tanja, Žel, Jana, Gruden, Kristina
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1569826/
https://ncbi.nlm.nih.gov/pubmed/16907967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-37
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