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Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

BACKGROUND: Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements. However, successful quantification depends crucially on the...

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Main Authors: Cankar, Katarina, Štebih, Dejan, Dreo, Tanja, Žel, Jana, Gruden, Kristina
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2006
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1569826/
https://ncbi.nlm.nih.gov/pubmed/16907967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-37
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