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Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates

BACKGROUND: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence...

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Bibliografski detalji
Izdano u:Microb Cell Fact
Glavni autori: Rautio, Jari, Barken, Kim Bundvig, Lahdenperä, Juhani, Breitenstein, Antje, Molin, Søren, Neubauer, Peter
Format: Artigo
Jezik:Inglês
Izdano: BMC 2003
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12780940/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1475-2859-2-4
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