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Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates

BACKGROUND: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence...

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Detalhes bibliográficos
Main Authors: Rautio, Jari, Barken, Kim Bundvig, Lahdenperä, Juhani, Breitenstein, Antje, Molin, Søren, Neubauer, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2003
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC156893/
https://ncbi.nlm.nih.gov/pubmed/12780940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-2-4
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