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DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate

Abstract Background Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the last decade, RNA extraction remains the most laborious and costly step in...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata, Atsushi J. Nagano
Format: Artigo
Sprache:Inglês
Veröffentlicht: BMC 2022-08-01
Schriftenreihe:Plant Methods
Schlagworte:
Direct-lysate reverse transcription
Targeted RNA-Seq
Reducing reagent
Gene expression analysis
Online-Zugang:https://doi.org/10.1186/s13007-022-00930-x
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