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Quantification of thioguanine-resistant lymphocytes from mice irradiated in vivo.

Adult mice were Co-60 gamma irradiated, and 7 months later splenocytes were isolated, cultured in microwells, and the frequency of hprt-deficient mutants was determined by measuring the cloning efficiency in media with 6-thioguanine. The mutant frequency at 2, 4, and 6 Gy was 1.6 x 10(-5), 4.4 x 10(...

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Autors principals: Lorenz, R, Göllner, T, Hempel, K
Format: Artigo
Idioma:Inglês
Publicat: 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1567986/
https://ncbi.nlm.nih.gov/pubmed/2272307
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