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Development of Group- and Serotype-Specific One-Step SYBR Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus

A quantitative one-step SYBR Green I-based reverse transcription (RT)-PCR system was developed for the detection and differentiation of four different dengue virus serotypes in acute-phase serum samples. A set of group- and serotype-specific primer pairs was designed against conserved sequences in t...

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Udgivet i:J Clin Microbiol
Principais autores: Shu, Pei-Yun, Chang, Shu-Fen, Kuo, Yu-Chung, Yueh, Yi-Yun, Chien, Li-Jung, Sue, Chien-Lin, Lin, Ting-Hsiang, Huang, Jyh-Hsiung
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Virology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156548/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12791857/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.6.2408-2416.2003
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