Development of Group- and Serotype-Specific One-Step SYBR Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus

A quantitative one-step SYBR Green I-based reverse transcription (RT)-PCR system was developed for the detection and differentiation of four different dengue virus serotypes in acute-phase serum samples. A set of group- and serotype-specific primer pairs was designed against conserved sequences in t...

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Detalhes bibliográficos
Main Authors: Shu, Pei-Yun, Chang, Shu-Fen, Kuo, Yu-Chung, Yueh, Yi-Yun, Chien, Li-Jung, Sue, Chien-Lin, Lin, Ting-Hsiang, Huang, Jyh-Hsiung
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC156548/
https://ncbi.nlm.nih.gov/pubmed/12791857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.6.2408-2416.2003
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