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Quantitative Detection of the Free-Living Amoeba Hartmannella vermiformis in Surface Water by Using Real-Time PCR

A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic...

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Bibliografiske detaljer
Main Authors: Kuiper, Melanie W., Valster, Rinske M., Wullings, Bart A., Boonstra, Harry, Smidt, Hauke, van der Kooij, Dick
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1563667/
https://ncbi.nlm.nih.gov/pubmed/16957190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00085-06
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