Investigating Mechanisms of Collagen Thermal Denaturation by High Resolution Second-Harmonic Generation Imaging

We apply the technique of second-harmonic generation (SHG) microscopy to obtain large area submicron resolution image of Type I collagen from rat tail tendon as it is heated from 40°C to 70°C for 0–180 min. The change in the collagen structure as reflected in its SHG image is observed at length scal...

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Detalhes bibliográficos
Main Authors: Sun, Yen, Chen, Wei-Liang, Lin, Sung-Jan, Jee, Shiou-Hwa, Chen, Yang-Fang, Lin, Ling-Chih, So, Peter T. C., Dong, Chen-Yuan
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Spectroscopy, Imaging, Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1562377/
https://ncbi.nlm.nih.gov/pubmed/16829565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.085902
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