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Nanosecond Responses of Proteins to Ultra-High Temperature Pulses

Observations of fast unfolding events in proteins are typically restricted to <100°C. We use a novel apparatus to heat and cool enzymes within tens of nanoseconds to temperatures well in excess of the boiling point. The nanosecond temperature spikes are too fast to allow water to boil but can aff...

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Detalhes bibliográficos
Main Authors: Steel, Bradley C., McKenzie, David R., Bilek, Marcela M. M., Nosworthy, Neil J., dos Remedios, Cristobal G.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1557564/
https://ncbi.nlm.nih.gov/pubmed/16844754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.090944
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