Novel Fluorescence Labeling and High-Throughput Assay Technologies for In Vitro Analysis of Protein Interactions

We developed and tested a simple method for fluorescence labeling and interaction analysis of proteins based on a highly efficient in vitro translation system combined with high-throughput technologies such as microarrays and fluorescence cross-correlation spectroscopy (FCCS). By use of puromycin an...

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Dettagli Bibliografici
Autori principali: Doi, Nobuhide, Takashima, Hideaki, Kinjo, Masataka, Sakata, Kyoko, Kawahashi, Yuko, Oishi, Yuko, Oyama, Rieko, Miyamoto-Sato, Etsuko, Sawasaki, Tatsuya, Endo, Yaeta, Yanagawa, Hiroshi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2002
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC155293/
https://ncbi.nlm.nih.gov/pubmed/11875038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.218802
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