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Cell-Free Synthesis of Encephalomyocarditis Virus

We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg(2+) and EMCV RNA concentrations. Excess input RNA impaired RNA replication and vi...

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Udgivet i:J Virol
Principais autores: Svitkin, Yuri V., Sonenberg, Nahum
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Replication
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154999/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12743313/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.77.11.6551-6555.2003
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